khtU [2018-04-10 10:35:46]

K⁺/H⁺ antiporter for K⁺ efflux

Locus: BSU09850

Isoelectric point: 9.29

Molecular weight: 43.79 kDa

Protein length: 408 aa

Gene length: 1224 bp

Function: potassium ion efflux

Product: K⁺/H⁺ antiporter for K⁺ efflux

Essential: no

Synonyms: yhaU

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Metal ion transporter
• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,059,203 → 1,060,420

Phenotypes of a mutant

• increased sensitivity to methylglyoxal PubMed

The protein

Structure

• 4BWZ (sodium proton antiporter NapA, 26% identity)

Localization

• cell membrane, integral membrane protein (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: khtS-khtT-khtU
  Description: PubMed

Biological materials

Mutant

• MGNA-A682 (yhaU::erm), available at the NBRP B. subtilis, Japan
• GP2078 (khtS-khtT-khtU::aphA3), available in Jörg Stülke's lab
• BKE09850 (ΔkhtU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAATACAAGATGGTCCATCG, downstream forward: _UP4_GGATAAAAAAAGCAGCACTG
• BKK09850 (ΔkhtU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAATACAAGATGGTCCATCG, downstream forward: _UP4_GGATAAAAAAAGCAGCACTG

FLAG-tag construct

• GP2432 khtU-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original publications

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